Review



targeted methylation sequencing platform  (Twist Bioscience)


Bioz Verified Symbol Twist Bioscience is a verified supplier
Bioz Manufacturer Symbol Twist Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Twist Bioscience targeted methylation sequencing platform
    Input and Output of each stage
    Targeted Methylation Sequencing Platform, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/Twist+Targeted+Methylation+Sequencing+Workflow/pmc13648300-370-14-13
    Average 94 stars, based on 1 article reviews
    targeted methylation sequencing platform - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices"

    Article Title: A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices

    Journal: Current Protocols

    doi: 10.1002/cpz1.70464

    Input and Output of each stage
    Figure Legend Snippet: Input and Output of each stage

    Techniques Used: Sequencing, Methylation, Analysis, Control, Tiling

    Estimated duration of the complete analysis
    Figure Legend Snippet: Estimated duration of the complete analysis

    Techniques Used: Analysis, QC/EQA/Proficiency, Sequencing, Methylation, Tiling

    Related Articles

    Sequencing:

    Article Title: Tissue-specific clonal selection and differentiation of CD4⁺ T cells during infection
    Article Snippet: .. A target sequence consisting of the TCR α and β genes separated by a self-cleaving P2A peptide was synthesized (Twist Bioscience) and used to replace the OTII TCR gene in the pMSCV-mCD4-PIG plasmid. .. The Platinum-E retroviral packaging cell line (RV-101, Cell Biolabs) was used for the viral packaging of pMSCV-mCD4-PIG TCR vectors generated by Twist.

    Synthesized:

    Article Title: Tissue-specific clonal selection and differentiation of CD4⁺ T cells during infection
    Article Snippet: .. A target sequence consisting of the TCR α and β genes separated by a self-cleaving P2A peptide was synthesized (Twist Bioscience) and used to replace the OTII TCR gene in the pMSCV-mCD4-PIG plasmid. .. The Platinum-E retroviral packaging cell line (RV-101, Cell Biolabs) was used for the viral packaging of pMSCV-mCD4-PIG TCR vectors generated by Twist.

    Plasmid Preparation:

    Article Title: Tissue-specific clonal selection and differentiation of CD4⁺ T cells during infection
    Article Snippet: .. A target sequence consisting of the TCR α and β genes separated by a self-cleaving P2A peptide was synthesized (Twist Bioscience) and used to replace the OTII TCR gene in the pMSCV-mCD4-PIG plasmid. .. The Platinum-E retroviral packaging cell line (RV-101, Cell Biolabs) was used for the viral packaging of pMSCV-mCD4-PIG TCR vectors generated by Twist.



    Similar Products

    guide rna grna sequences targeting hspb1  (Addgene inc)
    94
    Addgene inc guide rna grna sequences targeting hspb1
    Guide Rna Grna Sequences Targeting Hspb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/LSB-hsa-miR-299-5p+(Plasmid+%23103390)/pm42882493-70-0-19
    Average 94 stars, based on 1 article reviews
    guide rna grna sequences targeting hspb1 - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    targeted methylation sequencing platform  (Twist Bioscience)
    94
    Twist Bioscience targeted methylation sequencing platform
    Input and Output of each stage
    Targeted Methylation Sequencing Platform, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/Twist+Targeted+Methylation+Sequencing+Workflow/pmc13648300-370-14-13
    Average 94 stars, based on 1 article reviews
    targeted methylation sequencing platform - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    nebnext em seq kit  (Twist Bioscience)
    94
    Twist Bioscience nebnext em seq kit
    Input and Output of each stage
    Nebnext Em Seq Kit, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/NEBNext+EM-Seq+Kit+for+Twist+Targeted+Methylation+Sequencing/pmc13648300-370-7-13
    Average 94 stars, based on 1 article reviews
    nebnext em seq kit - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    targeted methylation sequencing kits  (Twist Bioscience)
    94
    Twist Bioscience targeted methylation sequencing kits
    Input and Output of each stage
    Targeted Methylation Sequencing Kits, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/NEBNext+EM-Seq+Kit+for+Twist+Targeted+Methylation+Sequencing/pm42633749-11965-10-9
    Average 94 stars, based on 1 article reviews
    targeted methylation sequencing kits - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    sgrna sequences targeting trmt6  (Addgene inc)
    96
    Addgene inc sgrna sequences targeting trmt6
    Input and Output of each stage
    Sgrna Sequences Targeting Trmt6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/lentiCRISPR+v2+(Plasmid+%2352961)/10__1186_slash_s40170___026___00452___3-88-0-13
    Average 96 stars, based on 1 article reviews
    sgrna sequences targeting trmt6 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    target sequences  (ATCC)
    96
    ATCC target sequences
    Input and Output of each stage
    Target Sequences, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/Xanthomonas+campestris+(Pammel)+Dowson+pathovar+campestris/10__1111_slash_ppa__70252-76-15-18
    Average 96 stars, based on 1 article reviews
    target sequences - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    shrna sequences targeting cherp  (Sangon Biotech)
    86
    Sangon Biotech shrna sequences targeting cherp
    <t>Cherp</t> serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using <t>shRNA.</t> (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.
    Shrna Sequences Targeting Cherp, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/sequences+shrna/pmc13255054-307-1-8
    Average 86 stars, based on 1 article reviews
    shrna sequences targeting cherp - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    non targeted metabolomic sequencing  (Novogene)
    86
    Novogene non targeted metabolomic sequencing
    <t>Cherp</t> serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using <t>shRNA.</t> (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.
    Non Targeted Metabolomic Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/analysis+metabolomic+non+targeted/pm42298429-90-32-28
    Average 86 stars, based on 1 article reviews
    non targeted metabolomic sequencing - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier
    targeted methylation sequencing workflow  (Twist Bioscience)
    94
    Twist Bioscience targeted methylation sequencing workflow
    <t>Cherp</t> serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using <t>shRNA.</t> (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.
    Targeted Methylation Sequencing Workflow, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/NEBNext+EM-Seq+Kit+for+Twist+Targeted+Methylation+Sequencing/pm42237736-96-9-8
    Average 94 stars, based on 1 article reviews
    targeted methylation sequencing workflow - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier
    twist targeted methylation sequencing workflow  (Twist Bioscience)
    86
    Twist Bioscience twist targeted methylation sequencing workflow
    <t>Cherp</t> serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using <t>shRNA.</t> (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.
    Twist Targeted Methylation Sequencing Workflow, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/target+sequence/human+methylome+panel+twist/pm42237736-92-8-13
    Average 86 stars, based on 1 article reviews
    twist targeted methylation sequencing workflow - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Input and Output of each stage

    Journal: Current Protocols

    Article Title: A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices

    doi: 10.1002/cpz1.70464

    Figure Lengend Snippet: Input and Output of each stage

    Article Snippet: Targeted methylation profiling was performed using the NEBNext EM‐seq Kit combined with the Twist Targeted Methylation Sequencing platform (Twist Bioscience, USA), following the manufacturer's instructions (New England Biolabs, ; “Twist Targeted Methylation Sequencing Protocol | Twist Bioscience,” accessed July 30, 2025).

    Techniques: Sequencing, Methylation, Analysis, Control, Tiling

    Estimated duration of the complete analysis

    Journal: Current Protocols

    Article Title: A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices

    doi: 10.1002/cpz1.70464

    Figure Lengend Snippet: Estimated duration of the complete analysis

    Article Snippet: Targeted methylation profiling was performed using the NEBNext EM‐seq Kit combined with the Twist Targeted Methylation Sequencing platform (Twist Bioscience, USA), following the manufacturer's instructions (New England Biolabs, ; “Twist Targeted Methylation Sequencing Protocol | Twist Bioscience,” accessed July 30, 2025).

    Techniques: Analysis, QC/EQA/Proficiency, Sequencing, Methylation, Tiling

    Cherp serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using shRNA. (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.

    Journal: iScience

    Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke

    doi: 10.1016/j.isci.2026.116257

    Figure Lengend Snippet: Cherp serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using shRNA. (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.

    Article Snippet: The shRNA sequences targeting Cherp were generated at Sangon Biotech.

    Techniques: Expressing, Biomarker Discovery, shRNA, Viability Assay, TUNEL Assay, Staining, Functional Assay, Imaging, Fluorescence